首页|期刊导航|中国农业科学（英文版）|Study on Enzymatic Hydrolysis of Gadus morrhua Skin Collagen and Molecular Weight Distribution of Hydrolysates
中国农业科学（英文版）2009，Vol.8Issue(6)：723-729,7.
Study on Enzymatic Hydrolysis of Gadus morrhua Skin Collagen and Molecular Weight Distribution of Hydrolysates
Study on Enzymatic Hydrolysis of Gadus morrhua Skin Collagen and Molecular Weight Distribution of Hydrolysates
摘要
Abstract
Process parameters on enzymatic hydrolysis and molecular weight (MW) distribution of collagen hydrolysates from Gadus morrhua skin were investigated.The optimal process parameters were obtained by the single-factor and orthogonal experiments.The molecular weight distribution of hydrolysates was determined using both Sephadex G25 partition and high speed liquid chromatography electricity spray mass spectrum (HPLC-ESI-MS).Collagen hydrolysates were first gained by an alkaline protease "alcalase" for 3 h at temperature (50℃),pH (10.0),substrate concentration (75 g L-1),and E/S (3%).The molecular weight distribution of collagen hydrolysates ranged from 300 to 1 500 Da,and most of peptides were under 1 200 Da.Sephadex G25 partition and HPLC-ESI-MS should be successfully employed to determine the molecular weight distribution of collagen hydrolysates.关键词
collagen hydrolysates/Gadus morrhua/molecular weight distribution/alkaline proteaseKey words
collagen hydrolysates/Gadus morrhua/molecular weight distribution/alkaline protease分类
农业科技引用本文复制引用
HUO Jian-xin,ZHAO Zheng..Study on Enzymatic Hydrolysis of Gadus morrhua Skin Collagen and Molecular Weight Distribution of Hydrolysates[J].中国农业科学（英文版）,2009,8(6):723-729,7.基金项目
The work was supported by Tianjin Committee of Science & Technology,China (06YFGZSH02300). （06YFGZSH02300）
