厦门大学学报（自然科学版）2001，Vol.40Issue(3)：726-734,9.
Hg2+、Cd2+对鱼类嗅觉的毒性及Ca2+的解毒作用
Study of structure-function alterations of olfactory organ in Hg2+、Cd2+ poisoned Tilapia. Sp and the detoxifcation of Ca2+
陈荣 1柴敏娟1
作者信息
- 1. 厦门大学环境科学研究中心,厦门大学海洋学系,
- 折叠
摘要
Abstract
When these fish (Tilapia. Sp) are exposed to four different kinds of Hg2+ or Cd2+ solutions, the structure-function alterations of olfactory organ are systematically studied by electrophysiological and histological method, and the detoxifcation of Ca2+ on Hg2+ or Cd2+ is also studied. The results are shown as follows: 1) Both of Hg2+ and Cd2+ are effective inhibitors on the EOG responses, and the inhibitions become stronger with the increasing of the ions concentration and prolonging of the polluted time. The IC50 (concentration which inhibited respoonses by 50%) values of Hg2+ and Cd2+ are 67.73 μg/L and 33.90 μg/L, respectively. The inhibitory effect of Cd2+ is stronger than that of Hg2+. 2) The inhibiting effects of the mixtures containing Ca2+ (80 mg/L)+Hg2+ or Ca2+ (80 mg/L) + Cd2+ weaker than that of single heavy metal ion with the same concentration, and the detoxifcation of Ca2+ on Hg2+-poisoned fish is much more pronounced than that of Cd2+. The results indicate that Ca2+ has some detoxifcation on the Hg2+ and Cd2+-poisoned fish. 3) After 15 days exposure to four polluting solution of 10、50 μg/L Hg2+ or Cd2+ respectively, the olfactory epithelium of all polluted fish are observed different degree damage by light microscopy. In the low concentration groups, the main alteration is the hypertrophy of mucous cells in the non-sensory region. In the high concentration groups, some vacuoles appear in the regional olfactory epithelium; some cells spill over the surface of olfactory epithelium from the region of dead sensory cells.
When the olfactory epithelium of Tilapia. SP is exposed to the mixture containing 80 mg/L Ca2+ and 10 μg/L Hg2+ or Cd2+, no changes is observed by light microscopy. But mixture groups containing 80 mg/L Ca2+ and 50 μg/L Hg2+ or Cd2+, olfactory epithelium has cellular shrinkage and no vacuole presented.关键词
Hg2+/Cd2+/Ca2+/罗非鱼/嗅觉/毒性/解毒分类
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陈荣,柴敏娟..Hg2+、Cd2+对鱼类嗅觉的毒性及Ca2+的解毒作用[J].厦门大学学报（自然科学版）,2001,40(3):726-734,9.
