首页|期刊导航|中国农业科学（英文版）|Introduction of rol Genes into Cotton (Gossypium hirsutum L.) Genome and Effects of Transgene Expression on the Plant Development
中国农业科学（英文版）2004，Vol.3Issue(10)：728-737,10.
Introduction of rol Genes into Cotton (Gossypium hirsutum L.) Genome and Effects of Transgene Expression on the Plant Development
Introduction of rol Genes into Cotton (Gossypium hirsutum L.) Genome and Effects of Transgene Expression on the Plant Development
摘要
Abstract
The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genome via Agrobacterium-mediated transformation. Molecular analyses and developmental identification of the putative transgenic plants were carried out by means of PCR, Southern blotting and field characterization. The results showed that the expression of rol genes greatly increased the rooting ability of the transgenic plants, and changed the plant development. Highly male-sterile plants with strong apical dominance and fertile plants with short internodes, stunted growth and improved economic characteristics were segregated from the T1 transgenic lines of wild rol B gene and the rol B gene driven by 35S promoter. The transgenic lines of rol ABC construct usually had normal boll setting and slow growth. Therefore we concluded that the rol genes, modified in suitable ways,could be used to create new cotton varieties with some highly valuable characteristics.关键词
Upland cotton/rol gene/Rooting loci/Root growth/Transformation/Developmental character/Agrobacterium rhizogenesKey words
Upland cotton/rol gene/Rooting loci/Root growth/Transformation/Developmental character/Agrobacterium rhizogenes引用本文复制引用
LIU Hai-yan,YANG Ye-hua,WU Zheng-bin,WANG Xue-kui,YAO Ming-jin..Introduction of rol Genes into Cotton (Gossypium hirsutum L.) Genome and Effects of Transgene Expression on the Plant Development[J].中国农业科学（英文版）,2004,3(10):728-737,10.基金项目
This investigation was partially supported by the National Natural Science Fundationof China(39970386) under the program Introduction of Transposon Tagging Systems into the cotton Genome and the Detection of Their Insertional Mutagenesis Efficiency. （39970386）
