中国临床康复2004，Vol.8Issue(32)：7322-7324,3.
创伤性休克大鼠血浆肾上腺髓质素和一氧化氮变化与组织器官保护的相关性
Correlation of the changes of blood adrenomedullin and nitric oxide with the protection of organ in rats with traumatic shock
孙高斌 1黄宗海 1黄绪亮 2厉周 1宋慧娟1
作者信息
- 1. 解放军第一军医大学珠江医院普通外科,广东省广州市,510282
- 2. 解放军第一军医大学全军休克微循环重点实验室,广东省广州市,510515
- 折叠
摘要
Abstract
BACKGROUND: Many inflammatory factors and nitric oxide(NO) are synthesized and released in traumatic shock, and play important roles in the process of shock.OBJECTIVE: To investigate the changes and correlation of adrenomedullin (AM) and nitric oxide(NO) in rats with traumatic shock.DESIGN: Randomized and controlled experimental study was adopted.SETTING and MATERIALS: The experiment was completed in Zhujiang Hospital of the First Military Medical University. Forty healthy SD rats, of either gender, weighing 280 - 300 g, were provided by the Experiment Animal Center of the First Military Medical University.INTERVENTION: Fourty Sprague-Dawley(SD) rats were randomly divided into control group(n= 10), traumatic shock without resuscitation group(n=10), traumatic shock on resuscitation group( n = 10) and aminpguandine(AG) group(n=10) . Ten rats as controls received cannulation after anesthesia. Thirty SD rats were made animal models of traumatic shock. Shafts of femurs of both sides were crashed and bled to mean arterial pressure(MAP) of 35 -45 mm Hg via femoral artery. Hypotention of rats in traumatic shock on resuscitation group were maintained 30 minutes,and then the lost blood was returned with an infusion with Ringer' s solution of the equal quantity. Rats of aminpguandine group were injected with aminpguandine 60 mg/kg. Then the changes of Plasma levels of AM and NO were observed.MAIN OUTCOME MEASURES: Changes and correlation of AM and NO after traumatic shock among the rats of the four groups.RESULTS: After the traumatic shock, the peak plasma level of AM at the 0.5 hour time point of the group without resuscitation and the group on resuscitation were (86. 89 ±2.23) and (143.88 ±2.74) ng/L respectively and the levels of NO were (47.88 + 2.29) and (86.56±2.15)μmol/L respectively, which were all higher than those of the control group. Changes were not significant in plasma level of AM and NO in aminpguandine group. The changes of concentration of AM were positively correlated with those of NO.CONCLUSION: AM and NO plays an important role in the pathological process of traumatic shock. The role played by AM in the process is probably through the mediation of NO.关键词
休克,创伤性/肾上腺髓质素/一氧化氮分类
医药卫生引用本文复制引用
孙高斌,黄宗海,黄绪亮,厉周,宋慧娟..创伤性休克大鼠血浆肾上腺髓质素和一氧化氮变化与组织器官保护的相关性[J].中国临床康复,2004,8(32):7322-7324,3.
