中国临床康复2004，Vol.8Issue(32)：7332-7334,3.
竹红菌乙素对视网膜和脉络膜光动力学治疗系统生物学效应的评定作用
Assessment of the biological effect of hypocrellin B in photodynamic therapy system for retina and choroid
摘要
Abstract
BACKGROUND: Benzoporphyrin derivative monoacid A ring (BPD-MA),as the photosensitizer in photodynamic therapy(PDT) for senile macular degeneration(SMD), can not be widely accepted in China because of its higher price. The biological effect of domestic photosensitizer hypocrellin B (HB) PTD system on retina and choroid was accessed in our study, to explore the worthiness of further study on the prospect of HB in treating SMD.OBJECTIVE: To introduce an experimental system to perform PDT irradiation system, the determination of the photosensitivity of the photosensitizers in vitro and evaluation of the therapeutic effect of photosensitizers for the retina and choroids in vivo.DESIGN: An open trial.SETTING, MATERIALS and INTERVENTION: The trial was conducted in the Department of Laser Medicine, General Hospital of Chinese PLA. Establishment of irradiation system: a laser window was created at the slit lamp microscope, which connected with 532 nm-laser in order to make the laser and the irradiation light of the slit lamp microscope to arrive at the fundus oculi at the same time. The photosensitivity of the photosensitizer was determined primarily by Methyl-thialolyl-tetrasolium(MTT) and compared with that of BPD-MA. Using chinchilla rabbits as subjects, the system was testified by domestic photosensitizer hypocrellin B photodynamic therapy (HB-PDT), whose biological effects at the irradiated area of rabbit were evaluated by fundus observation, fluorescein angiography and light and electron microscopy examination to investigate the feasibility and reliability of our experimental system.MAIN OUTCOME MEASURES: The coupling efficiency between sbt lamp microscope and laser, in vitro photosensitivity of photosensitizer and their biological effects at the irradiated area.RESULTS: The output of the laser was stable and the coupling efficiency between the laser and laser window of the slit lamp microscope was about 60%. The photosensitivity of HB in vitro was similar to BPD-MA, and HB-PDT displayed selective therapeutic property presented by closure of the retinal and choroidal capillaries with less damage to surrounding normal retina.CONCLUSION: It is useful to decide if a new photosensitizer fit for treating retinal and choroidal neovascularization by using the irradiation system and the method of determining the photosensitivity of the photosensitizer in vitro and biological effect in vivo.关键词
治疗学/光/光化学疗法/视网膜/脉络膜分类
医药卫生引用本文复制引用
韩丽娜,顾瑛,刘凡光,李晓松..竹红菌乙素对视网膜和脉络膜光动力学治疗系统生物学效应的评定作用[J].中国临床康复,2004,8(32):7332-7334,3.基金项目
全军医药卫生"十五"科研基金资助项目(01MB069) （01MB069）
