中国农业科学(英文版)2003，Vol.2Issue(7)：736-741,6.
Differences in Nuclear DNA Between Male-Sterile and Male-Fertile Lines of Sorghum bicolor
Differences in Nuclear DNA Between Male-Sterile and Male-Fertile Lines of Sorghum bicolor
FAN Chang-fa 1SUN Chun-yun 2GUO Xiao-cai 1NIU Tian-tang 1ZHANG Fu-yao3
作者信息
- 1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041 , P. R. China
- 2. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, P. R. China
- 3. Biotechnology Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031 , P. R. China
- 折叠
摘要
Abstract
Cytoplasmic male sterility (cms) is determined by nuclear-cytoplasmic interactions. Up tonow, most studies are focused on the comparison of cytoplasmic DNAs of male-sterile lines and male-fertilelines, and analysis of nuclear DNA has not been documented yet. In order to find out the possible difference innuclear genome of male-sterile line A1 Tx623 and corresponding male-fertile line Tx623 of sorghum, randomamplified polymorphic DNA (RAPD) approach was used to analyze their cytoplasmic and nuclear genomes.Total DNAs of them were amplified at first to screen primers, which were able to generate reproducible bandsspecific to male-sterile line or male-fertile line. Then the selected primers were used to amplify their mitochon-drial DNA (mtDNA) and chloroplast DNA (cpDNA). The origins of all the polymorphic fragments were ana-lyzed. After ruling out those amplified from cytoplasmic DNA, seventeen polymorphic fragments were deter-mined to be amplified from nuclear DNA. These fragments originated from nuclear DNA indicate that diffe-rences in sequence exist between the nuclear DNA of male-sterile line and male-fertile line of sorghum, whichdo not agree with the traditional standpoint that they have identical nucleus.关键词
Cytoplasmic male sterility/Sorghum bicolor/RAPD/Male-sterile line/Male-fertile line/Difference in nuclear DNAKey words
Cytoplasmic male sterility/Sorghum bicolor/RAPD/Male-sterile line/Male-fertile line/Difference in nuclear DNA引用本文复制引用
FAN Chang-fa,SUN Chun-yun,GUO Xiao-cai,NIU Tian-tang,ZHANG Fu-yao..Differences in Nuclear DNA Between Male-Sterile and Male-Fertile Lines of Sorghum bicolor[J].中国农业科学(英文版),2003,2(7):736-741,6.
