黑龙江八一农垦大学学报2009，Vol.21Issue(3)：73-75,94,4.
Protein A磁珠检测朊病毒方法的建立
The foundation of Protein A magnetism to detect Prion assay
李奇 1杨阳 1吕鹏 2宋博翠 1白宗瑞 1陈志宝1
作者信息
- 1. 黑龙江八一农垦大学生命科学学院,大庆,163319
- 2. 吉林大学中日联谊医院手术室科
- 折叠
摘要
Abstract
Objective The country need the fast and effective detection for Prion asthe high risk country where Scrapie and BSE may bresk out. The common assay of Western-Blot and Immunohistochemistry consume a long time and the associated experience is needed. Protein A magnetism has the feature of binding IgG differential.h could bind the IgG adequately,when mixing with polyclonal antibody.After intermixing with brain tissue protein digested by protein kinase, Prion could easily elute from Protein A magnetism.Interest protein band is 25KD through SDS-PAGE. When definiting the molecular weight of Priori,the result of detection is educed by SDS-PAGE electrophoresis. The assay is more handier and faster than common assay. The magnetism can reclaim and reuse,which reduce the cost.The assay of detection is a innovation and many branches could generally applicated it.关键词
Protein/A磁珠/朊蛋白(PrPC)/朊病毒(PrPSC)/免疫沉淀Key words
protein A magnetism/prp protein (PrPc)/Prion (PrP<'SC>)/immunoprecipitation分类
农业科技引用本文复制引用
李奇,杨阳,吕鹏,宋博翠,白宗瑞,陈志宝..Protein A磁珠检测朊病毒方法的建立[J].黑龙江八一农垦大学学报,2009,21(3):73-75,94,4.
