曲阜师范大学学报（自然科学版）2009，Vol.35Issue(3)：73-76,4.
拟康氏木霉产胞外多糖的分离纯化及组成初步研究
Separation, Purification and Component Research of an Extracellular Polysaccharide Produced by Trichoderma Pseudokoningii SDTP1
摘要
Abstract
A water-soluble extracellular polysaccharide (TPP-0) was obtained from Trichoderma pseudokoningii SDTP1, by deproteination using enzymolysis-Sevag method, and fractionation through Sephadex G-100 chromatography. Monosaccharide composition and the preliminary structure, molecular weight of TPP-0 were studied by ultraviolet (UV), infrared (IR), gas chromatography (GC), and high performance liquid chromatography (HPLC). TPP-0 had characteristic UV absorption peaks, being identical with normal polysaccharide. High performance gel permeation chromatography (HPGPC) was used to determine the molecular weight of TPP-0, and the result showed that the average peak molecular weight of TPP-0 amounted to 1.8×104. Three monosaccharides, i.e. rhamnose, glucose, galactose were detected by gas chromatography from the TPP-0 sample. And TPP-0 also contained glucuronic acid. The molar ratio of three monosaccharides was measured.关键词
拟康氏木霉/胞外多糖/分离纯化/紫外光谱/红外光谱/高效凝胶渗透色谱/气相色谱Key words
Trichoderma pseudokoningii/extracellular polysaccharide/isolation and purification/ultraviolet spectroscopy/infrared spectroscopy/high performance gel permeation chromatography/gas chromatography分类
农业科技引用本文复制引用
石磊..拟康氏木霉产胞外多糖的分离纯化及组成初步研究[J].曲阜师范大学学报（自然科学版）,2009,35(3):73-76,4.基金项目
曲阜师范大学科研基金资助(XJ200822). （XJ200822）
