中山大学学报(自然科学版)2001，Vol.40Issue(1)：73-76,4.
放线菌酮诱导SV40增强子的启动子活性的研究
Cydoheximide Induced Promoter Activity of SV40 Enhancer
王金发 1王宏斌 1何海琼 1刘兵 1冯冬茹1
作者信息
摘要
Abstract
The promoter activity of SV40 enhancer in the reporter vector of pCAT-Enhancer was induced by cycloheximide (CHX), a common protein synthesis inhibiter. This induced promoter activity can promote the CAT gene expression. The promoter activity of pCAT-Enhancer can be induced after treating 5 min and 30 ng/mL with CHX. In order to study whether the induced promoter activity of the pCAT-E by CHX is position-dependent. Two recombinant DNA has been constructed: pCAT-Bas-Enh ( - ) and pCAT-Bas-Enh ( + ), in which the position of SV40 enhancer element was changed. ThepCAT-Bas-Enh ( + )/( - )plasmids were used to transfect the CHO cell treated with CHX, no CAT activity detected.This result suggests that the CHX induced promoter activity of SV40 enhancer in pCAT-E is position-dependent.关键词
放线菌酮/SV40增强子/启动子/CHO细胞分类
生物科学引用本文复制引用
王金发,王宏斌,何海琼,刘兵,冯冬茹..放线菌酮诱导SV40增强子的启动子活性的研究[J].中山大学学报(自然科学版),2001,40(1):73-76,4.
