激光生物学报2006，Vol.15Issue(1)：73-78,6.
运用数字差异展示方法克隆一个人类新的锌指蛋白基因ZNF474
Identification of a Novel Human Zinc Finger Protein Gene ZNF474 by Digital Differential Display
摘要
Abstract
This study was designed to identify a novel testis overexpressed gene related to spermatogenesis by a new strategy of digital differential display (DDD). Based on the generation of expressed sequenced tags ( ESTs), comparing the testis libraries with other tissue or cell line libraries by the DDD program, we identified a new contig of the ESTs which were derived from testis libraries and represented a novel gene. A full-length cDNA sequence of the new gene named ZNF474 ( GeneBank accession number AY461732) by the HUGO Gene Nomenclature Committee in human testis was identified. ZNF474 was 1972 bp in length, located in chromosome 5q23.2. The sequence of the opening reading frame was 377 bp ～ 1471 bp, as was confirmed by RT-PCR and sequencing. The cDNA encodes a novel protein of 364amino acids protein that was 66 % identical to a mouse homologue. Northern blot analyses revealed that ZNF474 mRNA was exclusively expressed in testis and ovary and has one transcript. In situ hybridization revealed that ZNF474 high expressed in adult testis and seminoma. The brown granules of hybridization signal were found in spermatocyte, spermatids and spermatozoa, no expression in sertoli's cells and leydig's cells. We hypothesize that ZNF474 functions as a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development.关键词
数字差异展示方法/表达序列标签/ZNF474基因Key words
digital differential display/expressed sequence tag/ZNF474分类
生物科学引用本文复制引用
周畅,师建明,肖湘文,李麓芸,卢光琇..运用数字差异展示方法克隆一个人类新的锌指蛋白基因ZNF474[J].激光生物学报,2006,15(1):73-78,6.基金项目
National Natural Science Foundation of China ( 30500258 ) （ 30500258 ）
Doctor Startup Foundation of Hunan Normal University ( Sheng 050615 ) （ Sheng 050615 ）
