癌症2000，Vol.19Issue(8)：739-743,5.
尼卡地平和表皮生长因子对胶质瘤细胞生长的影响
Nicardipine inhibits growth-stimulating effect of epidermal growth factor on human glioblastoma cells
黄垂学 1胡守兴 2刘健 1王宇田 1陈兵 2谢季2
作者信息
- 1. 海南省人民医院神经外科,海南海口,570311
- 2. 湖南医科大学附二医院神经外科,湖南长沙,410011
- 折叠
摘要
Abstract
Objective: To observe and analyze the effects of epidermal growth factor (EGF) and the calcium channel antagonist nicardipine on the growth of U251MG, a human malignant glioma cell line, which possesses high-affinity EGF receptors. Methods:The growth effects of EGF and nicardipine on U251MG cultured in serum-free and serum-supplemented (10％ fetal bovine serum, FBS) medium respectively were observed by using MTT colorimeritric analysis. Results: ① EGF significantly enhanced the growth of U251MG cells in a concentration-dependent manner in serum-free medium. The maximal effect was seen at 20 ng/ml,which was approximated to those obtained in 10％ FBS. ② Nicardipine decreased U251MG cell proliferation, especially in serum-supplemented medium, and completely blocked the growth-stimulating effects of EGF. The combined effects of EGF (10ng/ml) and nicardipine equaled to those of nicardipine alone. Conclusions: Nicardipine can suppress the growth of U251MG cells and completely block the growth-stimulating effects of EGF,which suggests this drug could be a potential adjuvent therapeutic agent for human malignant gliomas.关键词
表皮生长因子/钙通道拮抗剂/尼卡地平/胶质瘤细胞Key words
Epidermal growth factor/Nicardipine/Calcium channel antagonist/Human malignant gliomas cell分类
医药卫生引用本文复制引用
黄垂学,胡守兴,刘健,王宇田,陈兵,谢季..尼卡地平和表皮生长因子对胶质瘤细胞生长的影响[J].癌症,2000,19(8):739-743,5.
