中华医学杂志（英文版）2002，Vol.115Issue(5)：740-742,3.
大鼠脊髓损伤后一氧化氮合酶基因表达的变化
Nitric oxide synthase gene expression in injured spinal cordtissue
摘要
Abstract
Objective To investigate gene expression of three nitric oxide synthase isozymes in injure d spinal cord tissue. Methods Thirty-six adult SD rats were randomly divided into six groups: a normal group and five injury groups, with six per each group. Animals in the injury groups w ere sacrificed at 2, 6, 12, 24, 48 h after injury. A compression injury model o n the spinal cord was made according to Nystrom B et al and gene expression of t he three NOS isozymes were examined by reverse transcription polymerase chain re action (RT-PCR). Results Gene expression of nNOS and eNOS were detectable in the normal group and were up -regulated quickly after injury, reaching a maximum at 6 h: (0.633±0.012) a nd (1.236±0.207). Gene expression of iNOS was detectable only in the injur y groups and it was gradually up-regulated after injury, reaching a maximum at 24 h:(1.043±0.049).Conclusion Injury to the spinal cord leads to early up-regulation of cNOS and late up-reg ulation of iNOS. Different NOS isozymes may play different roles in secondary s pinal cord injury.关键词
脊髓损伤/一氧化氮/一氧化氮合酶/基因表达Key words
spinal cord injury/nitric oxide/nitric oxide synthase/gene expression分类
医药卫生引用本文复制引用
刘成龙,靳安民,周初松,陈斌..大鼠脊髓损伤后一氧化氮合酶基因表达的变化[J].中华医学杂志（英文版）,2002,115(5):740-742,3.基金项目
This work was supported by the National Natural Sciences Foundation of China (No . 39800166). （No . 39800166）
