首页|期刊导航|中国农业科学（英文版）|Multiple Antibiotic Resistance and Heavy Metal Resistance Profile of Bacteria Isolated from Giant Freshwater Prawn (Macrobrachium rosenbergii) Hatchery
中国农业科学（英文版）2009，Vol.8Issue(6)：740-745,6.
Multiple Antibiotic Resistance and Heavy Metal Resistance Profile of Bacteria Isolated from Giant Freshwater Prawn (Macrobrachium rosenbergii) Hatchery
Multiple Antibiotic Resistance and Heavy Metal Resistance Profile of Bacteria Isolated from Giant Freshwater Prawn (Macrobrachium rosenbergii) Hatchery
摘要
Abstract
In this article,antibiogram and heavy metal resistance profile of bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) hatchery in Malaysia are described.Although giant freshwater prawn was introduced into Malaysia since the 1980s,there was no database information on antibiogram and heavy metal resistance profile of bacteria from giant freshwater prawn (M.rosenbergii) hatchery in Malaysia.Therefore,this study was carried out to determine the effectiveness of antibiotic and heavy metal resistance profile to control bacterial diseases in M.rosenbergii hatchery.The results can provide valuable information for local M.rosenbergii post-larval producer.Antibiotic sensitivity test was carried out by disk-diffusion method against 15 types of antibiotics as follows:oxolinic acid (2 μg),ampicillin (10 μg),erythromycin (15 μg),furazolidone (15 μg),lincomycin (15 μg),amoxicillin (25 μg),col istin sulphate (25 μg),doxycycline (30 μg),florfenicol (30 μg),flumequine (30 μg),nalidixic acid (30 μg),tetracycline (30 μg),oleandomyein (15 μg),fosfomycin (50 μg),and spiramycin (100 μg),whereas heavy metal resistance profile of the present bacterial isolates was determined by 2-fold agar dilution technique.In this study,5 types of bacteria were successfully isolated;they were Aeromonas spp.(n= 77),Escherichia coil (n = 73),Edwardsiella spp.(n = 62),Salmonella spp.(n= 75),and Vibrio spp.(n = 43).The result showed that furazolidone was the most effective antibiotic to control the bacteria isolated in this study,approximately 89.7% of the bacterial isolates were sensitive to this antibiotic.Multiple antibiotic resistance (MAR) index indicated that the hatchery water source and M.rosenbergii post-larval and sediment tanks were at high-risk exposure to the tested antibiotic.Furthermore,all the tested heavy metals (Cd2+,Cr6+ Hg2+,and Cu2+) failed to inhibit the growth of the bacterial isolates.Therefore,it indicated that the water source of the hatchery is contaminated with both antibiotic residues and heavy metal.关键词
Macrobrachium rosenbergii/hatchery/antibiotic/heavy metal/MAR indexKey words
Macrobrachium rosenbergii/hatchery/antibiotic/heavy metal/MAR index分类
农业科技引用本文复制引用
S W Lee,M Najiah,W Wendy,A Zahrol,M Nadirah..Multiple Antibiotic Resistance and Heavy Metal Resistance Profile of Bacteria Isolated from Giant Freshwater Prawn (Macrobrachium rosenbergii) Hatchery[J].中国农业科学（英文版）,2009,8(6):740-745,6.基金项目
This project was funded by Universiti Malaysia Terengganu and Ministry of Science,Technology and Innovation (MOSTI),Malaysia. （MOSTI）
