中国肺癌杂志2009，Vol.12Issue(7)：741-746,6.DOI:10.3779/j.issn.1009-3419.2009.07.003
肺癌细胞系中p120-catenin亚型1A和亚型3A对E-cadherin和β-catenin的调节功能
P120-catenin Isoforms 1A and 3A Differently Affect the Expression of E-cadherin and β-catenin in Lung Cancer Cells
摘要
Abstract
Background and objective Different isoforms of p120-catenin (p120ctn), the members of catenin family, are variably expressed in different tissues and cells. These isoforms may confer different properties with respect to cell adhesion and migration, but this concept needs experimental evidence. Methods To more precisely define their biological roles, we stably transfected isoforms 1A and 3A into the lung cancer cell lines A549 and NCI-H460, which had low expression of p120ctn. Using RT-PCR, Western Blot, and Transwell, we testified their impact on the expression ofE-cadherin, β-catenin, and the invasivenessof lung cancer cells. Results Expression of isoform 1A of p120ctn upregulated E-cadherin and β-catenin, as well as the ability of ceils to invasion. In contrast, overexpression of isoform 3A had a smaller influence on invasion. Conclusion P120ctn isoforms 1A and 3A may differently regulate the invasive, adhesive of hing cancer cells through distinct regulation of the expression of E-cadherin and β-catenin.关键词
肺肿瘤/p120-catenin/E-cadherin/肿瘤侵袭能力Key words
Lung neoplasms/p120-catenin/E-cadherin/Neoplasm invasiveness分类
医药卫生引用本文复制引用
方长清,刘洋,刘楠,张勇,王恩华..肺癌细胞系中p120-catenin亚型1A和亚型3A对E-cadherin和β-catenin的调节功能[J].中国肺癌杂志,2009,12(7):741-746,6.基金项目
本研究受国家自然科学基金(No.30670917和No.30870977)资助 （No.30670917和No.30870977）
