中华医学杂志（英文版）2007，Vol.120Issue(9)：743-748,6.
Inducing effects of hepatocyte growth factor on the expression of vascular endothelial growth factor in human colorectal carcinoma cells through MEK and PI3K signaling pathways
Inducing effects of hepatocyte growth factor on the expression of vascular endothelial growth factor in human colorectal carcinoma cells through MEK and PI3K signaling pathways
ZHANG Yu-hua 1WEI Wei 1XU Hao 1WANG Yan-yan 2WU Wen-xi1
作者信息
- 1. Department of Genaral Surgery, First Affiliated Hospital of Nanjing Medical University. Nanjing 210029, China
- 2. Immunology Research division, Department of pathology, Brigham Women's Hospital Boston, MA 02115, USA
- 折叠
摘要
Abstract
Background Vascular endothelial growth factor plays a key role in human colorectal carcinoma invasion and metastasis. However, the regulation mechanism remains unknown. Recent studies have shown that several cytokines can regulate the expression of vascular endothelial growth factor in tumor cells. In this study, we investigated whether hepatocyte growth factor can regulate the expression of vascular endothelial growth factor in colorectal carcinoma cells.Methods Hepatocyte growth factor and vascular endothelial growth factor in human serum were measured by ELISA.The mRNA level of vascular endothelial growth factor was analyzed by reverse transcription-PCR. Western blot assay was performed to evaluate levels of c-Met and several other proteins involved in the MAPK and PI3K signaling pathways in colorectal carcinoma cells.Results Serum hepatocyte growth factor and vascular endothelial growth factor were significantly increased in colorectal carcinoma subjects. In vitro extraneous hepatocyte growth factor markedly increased protein and mRNA levels of vascular endothelial growth factor in colorectal carcinoma cells. Hepatocyte growth factor induced phosphorylation of c-Met, ERK1/2 and AKT in a dose-dependent manner. Specific inhibitors on MEK and PI3K inhibited the hepatocyte growth factor-induced expression of vascular endothelial growth factor in colorectal carcinoma cells.Conclusion This present study indicates that hepatocyte growth factor upregulates the expression of vascular endothelial growth factor in colorectal carcinoma cells via the MEK/ERK and PI3K/AKT signaling pathways.关键词
hepatocyte growth factor/scatter factor/vascular endothelial growth factor/signaling pathway/receptor tyrosine kinases/colorectal carcinomaKey words
hepatocyte growth factor/scatter factor/vascular endothelial growth factor/signaling pathway/receptor tyrosine kinases/colorectal carcinoma分类
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ZHANG Yu-hua,WEI Wei,XU Hao,WANG Yan-yan,WU Wen-xi..Inducing effects of hepatocyte growth factor on the expression of vascular endothelial growth factor in human colorectal carcinoma cells through MEK and PI3K signaling pathways[J].中华医学杂志（英文版）,2007,120(9):743-748,6.
