中华医学杂志（英文版）2002，Vol.115Issue(5)：746-749,4.
肾癌特异相关新基因GYLZ-RCC18的克隆及功能研究
Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies
摘要
Abstract
Objective To clone the full length of renal cell carcinoma (RCC) related novel gene GYLZ -RCC18 and study its function.Methods SMART RACE technology was used to clone the full length of GYLZ-RCC18. RT-PCR was used to detect its expression in renal cell carcinoma tissue at different s tages and grades. We transfected the antisense oligonucleotide of GYLZ-RCC18 t o renal cell carcinoma cell line, GRC-1, and analyzed proliferation activity, g rowth rate, apoptosis, and mortality changes.Results The full length of GYLZ-RCC18 (GenBank accession number: BE825133) cDNA was abo ut 3.5kb. GYLZ-RCC18 had a higher expression in higher grades and stage s of renal cell carcinoma than in lower ones. The expression of GYLZ-RCC18 in rena l cell carcinoma was much higher than in normal kidney. After the transfection of GYLZ-RCC18 antisense oligonucleotide, the mortality of GRC-1 increased sig nificantly, while proliferative activity and growth rate were substantially inhi bited at the same time. The antisense oligonucleotide induced apoptosis of GRC -1 through the entire observation time.Conclusion GYLZ-RCC18 is an important novel gene related to renal cell carcinoma. Overexp ression of this gene results in higher growth and proliferative activity and has an antiapoptosis effect on renal cell carcinoma cells. Transfection of the ant isense oligonucleotide may inhibit the generation and development of renal cell carcinoma.关键词
肾肿瘤 癌 基因 GYLZ-RCC18Key words
kidney neoplasm/carcinoma/gene/GYLZ-RCC18分类
医药卫生引用本文复制引用
张强,陈琳,梁丽莉,席志军,丁义,佟明,张志文,李钟,郭应禄..肾癌特异相关新基因GYLZ-RCC18的克隆及功能研究[J].中华医学杂志（英文版）,2002,115(5):746-749,4.基金项目
This project was supported by a grant from the National Natural Science Foundati on of China (No. 39870841). （No. 39870841）
