中华医学杂志（英文版）2001，Vol.114Issue(7)：747-751,5.
bcl 10 基因在人肝细胞肝癌中的突变
bcl 10 gene mutation in hepatocellular carcinoma
程继华 1冷希圣 1蔡胜利 1曹宗献 1彭吉润 1王申五 1杜如昱 1曹广2
作者信息
- 1. 北京大学附属人民医院外科北京 100044
- 2. 首都医科大学附属安贞医院普外科北京 100029
- 折叠
摘要
Abstract
Abstract:Objective To detect the mutation frequency of the bcl 10 gene in the early and advanced stages of hepatocellular carcinoma (HCC).Methods Genome DNA samples were extracted from 46 cases of fresh HCC tumor tissues and their non-tumor adjacent tissues. Polymerase chain reaction-single strand conformation polymorphism method was used to detect point mutations of the three exons of the bcl 10 gene. For each individual exon, six random samples from those showing abnormal DNA bands were sequenced to verify those mutations. The relationship between serum alpha-fetoprotein (AFP) level and bcl 10 mutation, between the tumor size and bcl 10 mutation was also analyzed.Results Among the 46 samples, 26 cases (56.5%) were found to have mutations in exon 1, 5 out of the 6 cases were shown to have 5744 C→G mutation by sequencing; 25 cases (54.3%) were found to have mutations in exon 2, 4 out of the 6 cases were shown to have 11?311 T deletion mutation by sequencing. Twenty-one cases (45.7%) were found to have mutations in exon 3, all of the 6 cases selected for sequencing were shown to have 14?116 C→T mutation. Statistical analysis showed that neither serum alpha-fetoprotein level nor the size of hepatocellular carcinoma has a significant relationship with bcl 10 mutation.Conclusion The bcl 10 gene has a high mutation frequency in liver cancer.关键词
Bcl 10基因 肝癌 突变Key words
bcl 10 gene/liver cancer/mutation分类
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程继华,冷希圣,蔡胜利,曹宗献,彭吉润,王申五,杜如昱,曹广..bcl 10 基因在人肝细胞肝癌中的突变[J].中华医学杂志（英文版）,2001,114(7):747-751,5.
