中国肺癌杂志2009，Vol.12Issue(7)：747-752,6.DOI:10.3779/j.issn.1009-3419.2009.07.002
苦参碱和MAPK/ERK信号通路在抑制肺癌细胞诱导人脐静脉内皮细胞增殖和迁移中的作用
The Role of Matrine and Mitogen-Ativated Protein Kinase/Extracellular Signal-Regulated Kinase Signal Transduction in the Inhibition of the Proliferation and Migration of Hu-man Umbilical Veins Endothelial Cells Induced by Lung Cancer cells
摘要
Abstract
Background and objective Matrine, one of the major alkaloid components of the traditional Chinese medicine Sophora roots, has a wide range of pharmacological effects including anti-inflammatory activities, growth inhibition and induction of cell differentiation and apoptosis. Motigen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) has found to be a crucial signaling pathway in endothelial ceils. The aim of this study is to investigate the role of Matrine and MAPK/ERK signal transduction in the inhibition of the proliferation and migration of human umbilical veins en-dothelial cells (HUVECs) induced by lung cancer cells. Methods HUVECs were cultured with A549CM. Mat or PD98059 (i.e PD), specific inhibitor of MAPK/ERK, was added into the A549CM. The proliferation of the HUVECs was measured by cell counting. The migration of the HUVECs was observed by wound healing assay. The expression levels of ERK and p-ERK pro-tein were detected by Western Blot analysis. Results On 24 hours after intervention, the A549CM significantly stimulated the proliferation, migration and expression of p-ERK of HUVECs. Compared with the A549CM group, Mat significantly inhibited the proliferation, migration and p-ERK expression of HUVECs induced by A549CM. While PD only decreased the prolifera-tion and p-ERK expression of HUVECs induced by A549CM PD had no effect in the migration of HUVECs. Conclusion The results demonstrated that Mat and PD98059 can effectively decrease proliferation and expression ofp-ERK of HUVECs induced by A549CM. Furthermore Mat can also inhibit migration of HU-VECs induced by A549CM that did not changed by PD98059. These data implied that suppressing MAPK/ERK signal transduction may play the crucial role in resisting lung cacinoma anglo-genesis with Mat.关键词
肺肿瘤/苦参碱/丝裂原活化蛋白激酶/细胞外信号调节激酶/血管生成Key words
Lung neoplasms/Matrine/MAPK/ERK/Angiogenesis分类
医药卫生引用本文复制引用
卢进昌,罗强,程萍,刘先军,白明,涂明利..苦参碱和MAPK/ERK信号通路在抑制肺癌细胞诱导人脐静脉内皮细胞增殖和迁移中的作用[J].中国肺癌杂志,2009,12(7):747-752,6.基金项目
本研究受湖北省教育厅重点项目(No.B200524009)资助 （No.B200524009）
