中国农业科学2008，Vol.7Issue(1)：74-81,8.
The Metabolic Responses of Aspergillus flavus to N-Acetylcysteine, Ascorbate, and H2O2
The Metabolic Responses of Aspergillus flavus to N-Acetylcysteine, Ascorbate, and H2O2
摘要
Abstract
Aflatoxin, the secondary metabolite of Aspergillus flavus and A. Parasiticus, is the most toxic product in nature. In this study, N-acetylcysteine (NAC), ascorbate, and H2O2 were used to ascertain their effects on fungal metabolic esponse of A. Flavus. The results demonstrated that NAC did not affect fungal growth, but inhibited the aflatoxin B1 production, with the concomitant sporulation reduction. NAC increased the ratio of reduced glutathione (GSH) and oxidized glutathione (GSSG), hut decreased the activity of glutathione reductase (GR). Ascorbate had similar effect on fungal growth, sporulation,and GR activity, but GSH/GSSG and total glutathione (tGSH, including GSH and GSSG) were significantly increased. H2O2 at high concentration (5 mM) inhibited fungal growth, but the aflatoxin production was increased. At the same time, it reduced GR activity and enhanced tGSH. Though reductive agents had different effects on GSH metabolism, reductive conditions inhibited aflatoxin production and sporulation without any effect on fungal growth. The results in this report confirmed that the relationship between oxidative stress and aflatoxin production is theoretically important in controlling aflatoxin contamination.关键词
Aspergillus flavus, aflatoxin, glutathione metabolismKey words
Aspergillus flavus, aflatoxin, glutathione metabolism分类
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..The Metabolic Responses of Aspergillus flavus to N-Acetylcysteine, Ascorbate, and H2O2[J].中国农业科学,2008,7(1):74-81,8.基金项目
This work was financially supported by teh National Natural Science Foundation of China(30600408),and Natural Science Foundation of Hubei Province,China(2006ABA360). （30600408）
