中华医学杂志（英文版）2002，Vol.115Issue(5)：750-752,3.
内皮素-1是自发性高血压大鼠平滑肌细胞的有丝分裂剂
Endothelin-1, an important mitogen of smooth muscle cells of spontaneously hype rtensive rats
摘要
Abstract
Objective To study the features of vascular smooth muscle cell (VSMC) proliferation induce d by endothelin-1 (ET-1). Methods VSMCs of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats were cultu red and treated with ET-1. Basic fibroblast growth factor (bFGF) gene express ion was measured using both Northern blot and an enzyme-linked immunoassay. Results ET-1 resulted in an increase in bFGF transcripts at 8-24 h; bFGF levels were si gnificantly higher in VSMCs treated with ET-1 than in those not treated. Howev er, VSMCs gro wth responses in SHR and WKY were different. Smooth muscle cells of SHR were h yper-responsive to ET-1. Maximal bFGF mRNA levels were elevated 3.5-fold at 4 h of stimulation in WKY and 8-fold at 8h in SHR4. Moreover, the proliferatio n of VSMCs induced by ET-1 was inhibited by antisense phosphorothioate oligodeo xynucleotides (10 μmol/L AS-bFGF) but not sense bFGF oligomers at the same con centrations, being reduced by 80% in SHR and 40% in WKY vs control, respectively . Furthermore, the effect of AS-bFGF oligomers on SHR SMC proliferation is sig nificantly greater than on WKY SMC proliferation. Conclusion ET-1 may be required for exaggerated vascular growth responses in SHR and bFGF may be involved.关键词
内皮素-1/自发性高血压大鼠/平滑肌细胞/增殖Key words
endothelin-1/basic fibroblast growth factor/vascular smooth muscle cell/spontaneously hypertensive rat分类
医药卫生引用本文复制引用
刘革修,王华,欧大明,黄红林,廖端芳..内皮素-1是自发性高血压大鼠平滑肌细胞的有丝分裂剂[J].中华医学杂志（英文版）,2002,115(5):750-752,3.基金项目
This project was supported by the National Major Basic Program (G200056905), and National Natural Science Foundation of China (39470809). （G200056905）
