中华医学杂志（英文版）2002，Vol.115Issue(5)：753-758,6.
新发现的线粒体16S rRNA基因突变，3200T→C,与成人期发作二型糖尿病相关
Novel mitochondrial 16S rRNA mutation, 3200T→C, associated with adult-onset type 2 diabetes
摘要
Abstract
Objective To investigate the role of a potential diabetes-related mitochondrial region, w hich includes two previously reported mutations, 3243AG and 3316GA, in Chine se patients with adult-onset type 2 diabetes.Methods A total of 277 patients and 241 normal subjects were recruited for the study. M itochondrial nt 3116-3353, which spans the 16S rRNA, tRNAleu(UUR) and the NADH dehydrogenase 1 gene, were detected using polymerase chain reaction (PCR), direct DNA sequencing, PCR-restriction fragment length p olymorphism and allele-specific PCR. Variants were analyzed by two-tailed Fis her exact test. The function of the variants in 16S rRNA were predicted for mi nimal free energy secondary structures by RNA folding software mfold version 3.关键词
二型糖尿病 线粒体DNA 16S 核糖体RNA RNA二级结构模拟Key words
type 2 diabetes/mitochondrial DNA/16S ribosomal RNA/RNA secondary structure modeling分类
医药卫生引用本文复制引用
杨涛,林青云,曾文和,汤瑞芬,甘尔惠,陈婉珊,潘妙娟,巫向前,彭智培..新发现的线粒体16S rRNA基因突变，3200T→C,与成人期发作二型糖尿病相关[J].中华医学杂志（英文版）,2002,115(5):753-758,6.基金项目
This study was supported by Direct Grants from the Chinese University of Hong Ko ng (No. 2040585 and 2040661). （No. 2040585 and 2040661）
