中国水产科学2006，Vol.13Issue(5)：756-761,6.
鲢微卫星标记研制及其在鲢和鳙遗传多样性研究中的应用
Development of microsatellite DNA markers of silver carp (Hypophthalmichthys molitrix) and their application in the determination of genetic diversities of silver carp and bighead carp (Aristichthys nobilis)
摘要
Abstract
In order to evaluate the genetic diversity of silver carp(Hypophthalmichthys molitrix ) and bighead carp (Aristichys nobilis ) and determine their genetic differentiation, a GT microsatellite containing short fragment genomic DNA library of silver carp was constructed. Of 97 randomly selected and sequenced clones,87 contained a GT repeat motif. From 21 of them,22 pairs of primers were designed and used to investigate the polymorphism of 32 silver carp individuals and 7 bighead carp individuals collected from the Yangtze River basin, the most important spawning and hatching ground of these two fish species. All the markers developed amplify both silver carp and bighead carp DNAs. A total of 129 alleles were detected at 22 loci in the total sample. The number of alleles per locus ranges from 3 to 10 with an average of 5.9.All marker loci revealed high polymorphisms,with values ranging from 0.33 to 2.00(Shannon's diversity index) with the mean of 1.22.关键词
微卫星DNA标记/简单重复序列标记/鲢/鳙/遗传多样性/种间通用性Key words
microsatellite DNA marker/simple sequence repeat marker/Hypophthalmichthys molitrix/Aristichys nobilis/genetic diversity/cross-species amplification分类
生物科学引用本文复制引用
廖梅杰,杨官品,邹桂伟,危起伟,汪登强..鲢微卫星标记研制及其在鲢和鳙遗传多样性研究中的应用[J].中国水产科学,2006,13(5):756-761,6.基金项目
The Cooperative Project of The Key Lab of Freshwater Germplasm and Biotechnology of the Chinese Ministry of Agriculture ( LFB20040503 ) （ LFB20040503 ）
The National Natural Science Foundation of China(30471318). （30471318）
