中国林学（英文版）2006，Vol.8Issue(4)：75-81,7.
Determination of drought tolerance using root activities in Robinia pseudoacacia 'Idaho' transformed with mtl-D gene
Determination of drought tolerance using root activities in Robinia pseudoacacia 'Idaho' transformed with mtl-D gene
摘要
Abstract
Idaho locust (Robinia pseudoacacia 'Idaho') is an exotic multi-purpose tree used in landscaping, soil and water conservation, fodder sources and others. To improve its drought tolerance for reclaiming arid land, five lines of transformed mtl-D gene, as osmotic regulator in plant cells, have been selected and managed to determine their drought tolerance under experimental conditions.Qualitative and quantitative variables of transformed plants were studied. The critical value of drought tolerance was determined by detecting the 2,3,5-triphenyl tetrazolium chloride (TTC) reductants in roots and soil water content (SWC). The critical value for drought tolerance was SWC 6% while for the control plants the critical SWC was 8%; a moderate level of SWC is 13% and the highest SWC for plant endurance was 18%. The method proved to be reliable and sensitive in the evaluation of drought tolerance for forest trees.关键词
Robinia pseudoacacia 'Idaho', root activity, critical value of drought tolerance, soil water content, 2,3,5-triphenyl tetrazolium chloride (TTC)Key words
Robinia pseudoacacia 'Idaho', root activity, critical value of drought tolerance, soil water content, 2,3,5-triphenyl tetrazolium chloride (TTC)分类
农业科技引用本文复制引用
Wang Hua-fang,Zhu Yi-hong,Sun Hai-jun..Determination of drought tolerance using root activities in Robinia pseudoacacia 'Idaho' transformed with mtl-D gene[J].中国林学（英文版）,2006,8(4):75-81,7.基金项目
The study was supported by National High Technology Research and Development program of China (Grant No. 2001AA244041). （Grant No. 2001AA244041）
