中华医学杂志（英文版）2002，Vol.115Issue(5)：763-766,4.
蓝氏贾第鞭毛虫磷酸丙糖异构酶基因种内差异研究
The intraspecific difference of the triose phosphate isomerase (tim) gene from Giardia lamblia
卢思奇 1李继红 1张亚平 2文建凡 2王凤云1
作者信息
- 1. 首都医科大学细胞生物学系寄生虫学教研室,北京,100054
- 2. 中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明,650223
- 折叠
摘要
Abstract
Objective To investigate the intraspecific difference of the triose phosphate isomerase(ti m) gene from Giardia lamblia (G. lamblia).Methods Total genomic DNA of G. lamblia was extracted and partial fragments of the triose phosphate isomerase (tim) gene were amplified by polymerase chain reactio n (PCR). All nucleotide sequences were analyzed by using a phylogenetic analysi s, which was constructed with parsimony and Neighbor-joining (N-J) methods. Results A total of 124 variable sites (23% of all sequences detected) was defined, most of which were found at the silent sites of codons. Two similar phylogenetic tre es were constructed, subdividing 16 Giardia isolates into two groups. Conclusion The genetic diversity of G. lamblia appeared to be little affected by facto rs of both host and geography, while natural-selection played an important role in DNA molecular evolution level of the tim gene. The tim gene may be consider ed a very useful genetic marker of the population genetic structure of G. lam blia.关键词
蓝氏贾第鞭毛虫 磷酸丙糖异构酶(tim) 基因种内差异Key words
Giardia lamblia/triose phosphate isomerase (tim) gene/intraspeces difference分类
医药卫生引用本文复制引用
卢思奇,李继红,张亚平,文建凡,王凤云..蓝氏贾第鞭毛虫磷酸丙糖异构酶基因种内差异研究[J].中华医学杂志（英文版）,2002,115(5):763-766,4.
