中国肺癌杂志2009，Vol.12Issue(7)：765-769,5.DOI:10.3779/j.issn.1009-3419.2009.07.018
吉非替尼治疗前后肺腺癌患者血清蛋白质谱的变化
Analysis of Differentially Expressed Proteins in Self-Paired Sera of Advanced Non-small Cell Lung Cancer Patients Responsive to Gefinifib
摘要
Abstract
Background and objective All the advanced NSCLC patients that received EGFR-TKI therapy will eventually relapse after a period of efficacy. The aim of this study is to investigate the serum biomarkers as potential predictive factors for the efficacy of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) targeted therapy in advanced non-small cell lung cancer. Methods Twenty serf-paired serum samples were collected from 9 advanced NSCLC patients that evaluated as disease control (SD or PR) after gefinitib therapy, at the time points of before and after gefinitib treatment but 2 weeks before being evaluated as disease progress. AII samples were pre-separated by WCX microbeads, and then detected on the MALDI-TOF-MS platform of Bruker AutoflexTM. ClinProTools (Version: 2.1) was used to analyze the differentially expressed proteins. Results There were 7 protein peaks (m/z), 3 242.09, 8 690.36, 2 952.64, 3 224.04, 1 450.51, 1 887.8 and 3 935.73 found statistically differentially expressed between the self-paired samples. Three proteins (3 242.09, 2 952.64 and 3 224.04) were down-regulated and four proteins (8 690.36, 1 450.51, 1 887.8 and 3 935.73) up-regulated in gefinitib treated sera. Conclusion The data here suggest that several specific protein peaks might indicate gefinitib resistance, yet the identities of these proteins and the mechanisms underlying the responsiveness to gefinitib treatment need further investigation.关键词
肺肿瘤/表皮细胞生长因子受体/酪氨酸激酶抑制剂/血清蛋白/生物标记物/吉非替尼/基质辅助激光解吸电离飞行时间质谱Key words
Lung neoplasms/Epidermal growth factor receptor/Tyrosine kinase inhibitor/Serum protein/Biomarker/Gefitinib/Matrix-assisted laser desorption ionization time-of-flight mass spectrometry分类
医药卫生引用本文复制引用
杨学宁,黄迎,吴一龙,张绪超,杨衿记,黄玉娟,郭爱林,林嘉颖,安社娟,唐红艳,陈世良..吉非替尼治疗前后肺腺癌患者血清蛋白质谱的变化[J].中国肺癌杂志,2009,12(7):765-769,5.基金项目
本研究受广东省医学科研基金项目(No.A2005023)资助 （No.A2005023）
