农药学学报2000，Vol.2Issue(4)：76-78,3.
EPSP合成酶的纯化与制备
Purification and Preparation of EPSP Synthase
摘要
Abstract
The rapid purification(less than 1.5 h) of EPSP synthase from bean seedling by S ephadex G-50 and Mono-Q chromtography was reported. Specific activity of E PSP synthase obtained by the method was 175.2 nmol.min-1.mg-1.Conc entrated enzyme solution after adjusting to 50% glycerol(V/V) and 1mg.mL -1BSA, was stored at -20℃. EPSP synthase activity was stable at least f or 150 days.The activity of EPSP synthase was inhibited approximately 50% by 6.3 μmol.L-1 glyphosate. It showed that the purified EPSP synth ase as herbicide screening model is possible. This purified method has been used to study enzyme mechanism of the glyphosate resistant bean.关键词
菜豆/EPSP合成酶/纯化/制备Key words
EPSP synthase/Purification/Preparation分类
化学化工引用本文复制引用
向文胜,王相晶,覃兆海,任天瑞,张雅莉,张文吉,苏少泉..EPSP合成酶的纯化与制备[J].农药学学报,2000,2(4):76-78,3.基金项目
国家自然科学基金(39670498)资助项目 （39670498）
