肝胆胰外科杂志2001，Vol.13Issue(2)：76-78,3.
从胆汁中检测p53基因突变诊断胆囊癌
Detection of point mutations in the p53 gene in bile for diagnosis of gallbladder cancer
金晓凌 1王炳生 2井清源1
作者信息
- 1. 南京医科大学附属南京第一医院 普外科,
- 2. 复旦大学附属中山医院 普外科,
- 折叠
摘要
Abstract
Objective：To investigate the value of detection of point mutations in the p53 gene in bile for diagnosis of gallbladder cancer(GBC).Methods：Genetic alteration of the p53 gene was examined in bile specimens of 15 GBC cases and 10 cases of benign gallbladder disease using PCR(polymerase chain reaction)-SSCP(single-strand conformation polymorphism) combined with silver staining. Cytologic diagnosis was performed with all of bile specimens.Results：Sensitivity and specificity of bile cytologic diagnosis for GBC were 13% and 100% respectively. The p53 gene mutation was detected in nine of the 15 GBC cases examined(60%), and not in all of 10 benign bile specimens. The DNA analysis demonstrated the presence of p53 gene mutations in 8 cases of GBC with false-negative cytologic diagnosis.Conclusion：Detection of point mutations in the p53 gene in bile is highly specific for diagnosing GBC and may be a valuable diagnostic modality for GBC.关键词
胆囊肿瘤/诊断/胆汁/p53基因分类
医药卫生引用本文复制引用
金晓凌,王炳生,井清源..从胆汁中检测p53基因突变诊断胆囊癌[J].肝胆胰外科杂志,2001,13(2):76-78,3.
