生物技术通报Issue(7)：76-81,6.
OsbHLH1基因过表达载体构建及转化水稻研究
Construction of Over-expression Vector of OsbHLH1 Gene and Transformation of Rice
摘要
Abstract
OsbHLH1 gene which codes one of transcription factors of the bHLH family,is involved in cold tolerance. An over-expression vector including OsbHLH1 gene and Bar gene was constructed based on pCAMBIA3300. The target genes were transformed into Dongdao number 3 by particle bombardment. Forty seven plantlets were regenerated.Nine of them were analyzed by PCR and Southern blotting, and the results showed that the target genes had been integrated into the rice genome. The spreading of glufosinate on the leaves indicated that the Bar gene had been expressed normally in rice. Under low temperature treatment, the changes of net photosynthetic rate of the regenerated plantlets, except plantlet number 45, were significantly lower than that of check, which showed that over-expression of OsbHLH1 gene could be able to enhance the cold tolerance of rice.关键词
OsbHLH1基因/Bar基因/过表达/基因枪转化/水稻/耐低温/光合速率Key words
OsbHLH1 gene Bar gene Over-expression Particle bombardment Rice Cold tolerance Photosynthetic rate分类
农业科技引用本文复制引用
罗伯祥,邓力华,陈芬,陈受宜,李文彬,肖国樱..OsbHLH1基因过表达载体构建及转化水稻研究[J].生物技术通报,2009,(7):76-81,6.基金项目
转基因生物新品种培育科技重大专项(2008ZX08001-003),国家科技支撑计划区域项目(2007BAD77B03),中国科学院知识创新工程重大项目(KSCX1-YW-03) （2008ZX08001-003）
