首页|期刊导航|中国农业科学（英文版）|The Susceptibilities of Oxya chinensis (Orthoptera: Acridoidea) to Malathion and Comparison of the Esterase Properties from Three Collected Populations in Tianjin Area, China
中国农业科学（英文版）2009，Vol.8Issue(1)：76-82,7.
The Susceptibilities of Oxya chinensis (Orthoptera: Acridoidea) to Malathion and Comparison of the Esterase Properties from Three Collected Populations in Tianjin Area, China
The Susceptibilities of Oxya chinensis (Orthoptera: Acridoidea) to Malathion and Comparison of the Esterase Properties from Three Collected Populations in Tianjin Area, China
摘要
Abstract
The susceptibilities of Oxya chinensis to malathion were studied in three populations collected from outskirt of Tianjin, China, using bioassays and biochemical analysis. Populations were chosen to represent different exposure to insecticides: BDG (Beidagang; low exposure), BD (Baodi; high exposure previously but low exposure now), and JN (Jinnan; high exposure). The results showed that the LD50 values of BD and JN populations were 3.95-and 12.02-fold and 3.64- and 10.07-fold higher than that of BDG population in females and males, respectively. The LD50 values in females were higher than those in males. The results of biochemical analysis indicated that the esterase (EST) activities in JN population were higher than those in BD and BDG populations. They showed that when α-NA, α-NB, and β-NA were used as substrates, females' EST activities of JN population were 1.11-, 1.30-, and 1.14-fold and 1.39-, 1.59-, and 1.54-fold higher than those of BD and BDG populations, respectively. When α-NA, α-NB, and β-NA were used as substrates, males' EST activities of JN population were 1.13-, 1.12-, and 1.00-fold and 1.20-, 1.14-, and 1.07-fold higher than those of BD and BDG populations, respectively. The results also showed that the specific activities of the females were higher than those of the males in the BD and JN populations, whereas the specific activities of the males were higher than those of the females in the BDG population. The results of bioassay were consistent with those of biochemical analysis. Thus, it was inferred that the elevated ESTs activities might play an important role in conferring the differences of susceptibility of O. Chinensis to malathion in the three collected populations and between the females and the males. The observed changes in the kinetic parameters might be explained by differential expression patterns of isozymes so that the insect esterases have different affinities and maximum velocities toward the same substrate.关键词
Oxya chinensis/esterase/enzyme kinetics/malathion susceptibilityKey words
Oxya chinensis/esterase/enzyme kinetics/malathion susceptibility分类
农业科技引用本文复制引用
WU Hai-hua,YANG Mei-ling,GUO Ya-ping,MA En-bo..The Susceptibilities of Oxya chinensis (Orthoptera: Acridoidea) to Malathion and Comparison of the Esterase Properties from Three Collected Populations in Tianjin Area, China[J].中国农业科学（英文版）,2009,8(1):76-82,7.基金项目
The authors thank Professor Zhu Kunyan (Kansas State University, USA) for providing instructions in the course of experiments. This work was supported by the National Natural Science Foundation of China (30570247)and the Science and Technology Commission of Shanxi Province, China (2006011075). （Kansas State University, USA）
