首页|期刊导航|中国农业科学(英文版)|Vegetative Compatibility and Asexual Recombination in Magnaporthe grisea from Jiangsu Province, China
中国农业科学(英文版)2003，Vol.2Issue(7)：768-772,5.
Vegetative Compatibility and Asexual Recombination in Magnaporthe grisea from Jiangsu Province, China
Vegetative Compatibility and Asexual Recombination in Magnaporthe grisea from Jiangsu Province, China
摘要
Abstract
Vegetative compatibility among isolates of different races in Magnaporthe grisea collectedfrom Jiangsu Province and asexual recombination among compatible isolates by anastomosis were tested.Twenty isolates involving seven races from diseased rice plants were paired on polished rice rose bengal mediumand incubated at 25℃ in darkness for 18 days. Among 173 pairings tested, solid hyphai fusion lines formed byanastomosis between 124 pairings, indicated that these isolates were vegetative compatible with each other.The result showed that most M. grisea isolates were vegetative compatible. Furthermore, 17 vegetative com-patible pairings between monoconidial isolates with MBCsIPTr marker and isolates with MBCrIPTs markerwere selected to detect the asexual recombination between the compatible isolates of different races. The asexu-al recombinants with MBCrIPTr marker were detected in single hyphal fragment progenies in thirteen of theseventeen pairings. The percentage of recombinants was about 0.6 - 11.3%. Results showed that vegetative com-patibility was prevailing among isolates of M. grisea in Jiangsu Province in vitro. These results also suggested thatasexual recombination may be an important mechanism for M. grisea to maintain genetic diversity in nature.关键词
Magnaporthe grisea/Vegetative compatibility/Asexual recombinationKey words
Magnaporthe grisea/Vegetative compatibility/Asexual recombination引用本文复制引用
WANG Yan-li,ZHANG Zheng-guang,ZHENG Xiao-bo..Vegetative Compatibility and Asexual Recombination in Magnaporthe grisea from Jiangsu Province, China[J].中国农业科学(英文版),2003,2(7):768-772,5.基金项目
The work was supported in part by the National Natural Science Foundation of China (39970026), Backbone Teacher of High School Fund, Trans-century Training Program Foundation for the Talents by the State Education Commission and the National 863 Program, （39970026）
