中国病理生理杂志2009，Vol.25Issue(4)：774-780,7.
人骨髓源干细胞向胰岛素分泌细胞分化
Differentiation of human bone marrow-derived stem cells into insulin-producing cells in vitro and in vivo
摘要
Abstract
AIM: To explore the possibility of differentiating functional insulin-producing cells from human BM-derived stem cells. METHODS: Mesenchymal stem cells were isolated from human bone marrow. Then these cells were induced with epidermal growth factor, β-mercaptoethanol and high concentration of glucose. The gene expression related to islet β cells was detected by RT-PCR. Insulin in the treated cells was examined by immunocytochemistry. In addition, the levels of insulin secretion and glucose-stimulated insulin release were examined by microparticle enzyme immunoassay. Finally, the induced cells were implanted into the right renal subcapsular space of diabetic mice. Blood glucose levels were monitored 16 d after implantation. The right kidneys of the treated mice were harvested for immunohistochemistry. RESULTS: The key genes related to pancreatic β cells had been confirmed to express by PCR and insulin was detected by immunocytochemistry in differentiated human BM-derived stem cells induced by high glucose, which responded to glucose challenge. Furthermore, implantation of the cells in renal subcapsular space was able to lower the glucose levels in hyperglycemic mice. After 16 days, the implanted cells were determined still to be insulin positive cells by immunohistochemistry. CONCLUSION: These results indicate human BM-derived stem cells are capable of differentiating into functional insulin-producing cells and may represent a pool of cells for the treatment of diabetes.关键词
人类/骨髓/干细胞/细胞分化/胰岛素分泌细胞Key words
Human/Bone marrow/Stem cells/Cell differentiation/Insulin-producing cells分类
生物科学引用本文复制引用
陆琰,张洹,迟作华..人骨髓源干细胞向胰岛素分泌细胞分化[J].中国病理生理杂志,2009,25(4):774-780,7.基金项目
Supported by a Foundation of the Key Discipline Construction from Oversea Chinese Affairs Office of the State Council of the People's Republic of China (No.2005-10) （No.2005-10）
