现代妇产科进展2004，Vol.13Issue(1)：77-79,3.
The effect of interleukin-6 gene transfer on human cord blood megakaryopoiesis
The effect of interleukin-6 gene transfer on human cord blood megakaryopoiesis
Yang Xingsheng 1Hitoshi Kurata 2Kazuyuki Fujita 2Kenichi Tanaka2
作者信息
- 1. Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University 250012 China
- 2. Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Science,Japan
- 折叠
摘要
Abstract
Objective:To investigate the effect of IL-6 gene transfer into human cord blood hematopoietic stem cells on the production of megakaryocytic progenitors. Methods: IL-6 gene was transfected into human cord blood CD34 + cells using a retrovirus vector with the aid of recombinant fibronectin fragments in the presence of a cocktail of cytokines (SCF, IL-6, sIL-6R, FL, and TPO). Colony-forming units-megakaryocyte (CFU-MK) assays were perfonned as IL-6 gene transduced CD34 + cells were incubated alone or in combination with IL-3 or sIL-6R, controlled with neoR gene transduced CD34 + cells. Results: IL-6 alone or sIL-6R alone stimulated few CFU-MK colonies, the addition of sIL-6R to IL-6 gene transduced CD34 + cells significantly enhanced the production of CFU-MK colonies. IL-6 gene transduced CD34 + cells showed a modest synergistic effect with IL-3. Conclusion: These results suggest that IL-6 gene transfer may protect patients from chemotherapy-induced thrombocytopenia.关键词
Interleukin-6/Gene transfer/Human cord blood/MegakaryocytesKey words
Interleukin-6/Gene transfer/Human cord blood/Megakaryocytes分类
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Yang Xingsheng,Hitoshi Kurata,Kazuyuki Fujita,Kenichi Tanaka..The effect of interleukin-6 gene transfer on human cord blood megakaryopoiesis[J].现代妇产科进展,2004,13(1):77-79,3.
