分析测试学报2001，Vol.20Issue(2)：77-79,3.
铕-芦氟沙星的氧化产物- EDTA 配合体系的敏化荧光
Fluorescence System of Eu3＋ － Oxidation Product of Rufloxacin－ EDTA and Its Application
刘建群 1赵慧春2
作者信息
- 1. 萍乡高等专科学校化工系,
- 2. 北京师范大学化学系,
- 折叠
摘要
Abstract
Rufloxacin can be oxidized by H2O2 in HAc solution at 100 ℃ ,and its fluorescence is lost.A ternary complex of the oxidation product with EDTA and Eu3＋ can be formed in the HAc－ NaAc buffer solution.The complex has characteristic fluorescence of Eu3＋ at an emission wavelength of 617 nm(λ ex=352 nm).The linear relationship between fluorescence intensity and concentration of rufloxacin in the range of 5.0× 10－ 8～ 2.5× 10－ 6 mol/L was found.A method for the determination of rufloxacin in human urine was establised by Eu3＋ sensitized fluorescence analysis in view of the above.Under the selected optimum conditions,the detection limit of the method is 1.5× 10－ 8 mol/L.The method is simple,rapid and sensitive.The mechanism of the oxidation reaction of rufloxacin in HAc solution was also discussed according to the changes of UV,fluorescence and infrared spectra.关键词
芦氟沙星/氧化反应/铕/敏化荧光分类
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刘建群,赵慧春..铕-芦氟沙星的氧化产物- EDTA 配合体系的敏化荧光[J].分析测试学报,2001,20(2):77-79,3.
