华西口腔医学杂志2001，Vol.19Issue(2)：77-79,82,4.
家兔颌面部爆炸伤早期血管内皮细胞生长因子及碱性成纤维细胞生长因子的变化和作用
Study of the Changes and Effect of VEGF and bFGF in Early
Stage of Maxillofacial Blast Injury in Rabbits
张从纪 1李慧增 1杨军 1周树夏2
作者信息
- 1. 第三军医大学西南医院口腔科,
- 2. 第四军医大学口腔医学院
- 折叠
摘要
Abstract
Objective:The aim of this study is to determine the content of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in the wound fluid during maxillofacial soft tissue blast injury, and to evaluate their effects on angiogenesis of wound healing.Methods: The rabbit model of maxillofacial blast injury was made by KTY_04 blasting cap. The content of VEGF and bFGF in wound fluid which was collected by using polyving alcohol (PVA) sponge, were detected by ELISA. The distributions of VEGF and bFGF in the wound tissue were observed by using the techniques of in situ hybridization and immunohistochemistry.Results: In the wound fluid of maxillofacial blast injury, the content of VEGF was rising steadily at the first week after injury. Comparing with natural sera, it showed difference from the first day after injury, and showed obvious different from the third day (P<0.01), on which the content was 2.9 ng/ml±2.7 ng/ml. It reached the peak on the seventh day after injury. The bFGF level in wound fluid was 565 pg/ml±436 pg/ml, the peak value was gainged at the sixth hour after injury. In the following days, it declined close to the serum level three to five days after injury, and then moved up slightly on the seventh day after injury. The expression of VEGF was located in epithelial cells, vascular endothelial cells and some chronic inflammatory cells. The mRNA transcription of bFGF was present in fibroblasts, endothelial cells and macrophages.Conclusion: VEGF and bFGF take part in two angiogenic cascades with synergistic action in maxillofacial blast injury. The bFGF stimulates the initial angiogenesis and induces the production of VEGF. The subsequent and more prolonged angiogenic stimuli can be mediated by VEGF.关键词
颌面部爆炸伤/血管内皮生长因子/碱性成纤维细胞生长因子分类
医药卫生引用本文复制引用
张从纪,李慧增,杨军,周树夏..家兔颌面部爆炸伤早期血管内皮细胞生长因子及碱性成纤维细胞生长因子的变化和作用[J].华西口腔医学杂志,2001,19(2):77-79,82,4.
