农业生物技术学报2001，Vol.9Issue(1)：77-80,4.
枯草芽孢杆菌SO113分泌蛋白的抑菌作用及抗菌蛋白的分离纯化
The Antibacterial Effect of the Secreted Peptide from Bacillus subtilis SO113 and Separation and Purification of the Antibacterial Peptides
林东 1徐庆 1刘忆舟 1魏军明 1瞿礼嘉 1顾红雅 1陈章良1
作者信息
摘要
Abstract
In in vitro tests,Bacillus subtilis SO113 showed strongly antibacterial activity against Xanthomonas oryzae pv.oryzae(Xoo)(showing a clear inhibition zone,anti-Xoo).A crude antibacterial preparation(CAP)was obtained by adding ammonium sulphate to supernatant fluid of B.subtilis SO113 to a final saturation of 60% and displayed strong killing activity to 7 pathotypes of Xoo which covers all rice cropping-region in China.The anti-Xoo activity of CAP is thermostable,resistant to p
roteinase E,and partially sensitive to proteinase K.One and three peaks with anti-Xoo activity were obtained from CAP, which previously heated at 100℃ for 15 min and salts was removed from, after DEAE-Sepharose Fast Flow and CM-Sepharose Fast Flow, respectively. It is significant to clone the genes encoding those anti-Xoo peptides because the anti-Xoo peptides from B.subtilis SO113 showed extensively inhibitory activities against 7 pathotypes of Xoo in China.关键词
枯草芽孢杆菌SO113/抗菌蛋白/水稻白叶枯病菌/分离纯化分类
农业科技引用本文复制引用
林东,徐庆,刘忆舟,魏军明,瞿礼嘉,顾红雅,陈章良..枯草芽孢杆菌SO113分泌蛋白的抑菌作用及抗菌蛋白的分离纯化[J].农业生物技术学报,2001,9(1):77-80,4.
