陕西科技大学学报（自然科学版）2007，Vol.25Issue(3)：77-81,5.
产4-α-葡萄糖基转移酶链霉菌的筛选
SCREENING OF Streptomyces WITH 4-α-GLUCANOTRANSFERASE
王水兴 1郭勇 2许杨 1李燕萍2
作者信息
- 1. 华南理工大学生物科学与工程学院,广东,广州,510641
- 2. 南昌大学中德联合研究院,江西,南昌,330047
- 折叠
摘要
Abstract
To screen the strains from Streptomyces with 4-α-glucanotransferase activities. The detailed action profile of crude enzyme from Streptomyces on amylose was analyzed and tested whether this crude enzyme could catalyze cyclization of amylose. The mixture solution that was treated by crude enzyme resulted in a rapid decrease of absorbance of iodine-amylose complex and the accumulation of a product that was resistant to an exo-amylase (glucoamylase) but was degraded by an endo-type α-amylase to glucose and maltose. These results indicated that the crude enzyme catalyzed cyclization of amylose to produce cycloamylose. These results demonstrated that there were 4-α-glucanotransferase in the crude enzyme.关键词
4-α-葡萄糖基转移酶/大环糊精/链霉菌/麦芽糖转糖基酶Key words
4-a-glucanotransferase/ cycloamylose/ Streptomyces/ amylomaltase分类
生物科学引用本文复制引用
王水兴,郭勇,许杨,李燕萍..产4-α-葡萄糖基转移酶链霉菌的筛选[J].陕西科技大学学报（自然科学版）,2007,25(3):77-81,5.
