摘要
Abstract
BACKGROUND: It has been indicated that neuro-immuno-modulation peptide, α-melanophore-stimulating hormone(α-MSH), can play an effective role in anti-inflammation, antibiosis, defervescence and immunological regulation through inhibiting the release of proinflammatory factor, improving the vigor of lymphocyte and other ways, so that it has a good prospect in the prevention and treatment of systemic inflammatory response syndrome.OBJECTIVE: To study the influence of α-MSH on the infiltration of cerebral inflammatory cell and the expression of intercellular adhesion molecule (ICAM-1) following ischemia-reperfusion.DESIGN: Randomly double-blind and control experiment.SETTING: Emergency Department of Southwest Hospital of the Third Military Medical University of Chinese PLA.MATERIALS: Sixty healthy male Wistar rats provided by Animal Center of the Third Military Medical University of Chinese PLA, were divided into three groups including sham operated group, ischemia group and α-MSH treated group, and each group was divided into five subgroups according to reperfusion for 6, 12, 24,48,72 hours after ischemia of 2 hours with 4 rats per phase in each group.INTERVENTIONS: Models of middle cerebral artery occlusion were prepared. Two rats were detected for the expression of ICAM-1 in each phase with immunohistochemical method. And two rats were detected for the activity of myeloperoxidase(MPO) in each phase. Ultrastructures of brain tissue cells were observed with transmission electron microscope.MAIN OUTCOME MEARSURES: Positive expression of ICAM-1, MPO activity and cellular ultrastructure.RESULTS: Positive expression cell population of ICAM-1 per square millimeter in brain tissue slice of rats under high-power lens( ×200) after ischemia-reperfusion was stable at(1.02 ±0. 14) - (1.15 ±0. 16)/mm2 in sham operated group, which began to increase after 6 hours in ischemia higher than that of the sham operated group while ICAM-1 expression was significantly down regulated in α-MSH treatment group form(4 51 ±0 11 )/mm2to(2.97 ±0.09)/mm2. MPO activity in brain was stable at(3.17 ±0.27) -(3.67 ±0. 23) nkat in sham operated group, which began to increase at 12hours in ischemia group, and reached peak level at 24 hours from (5.83±0. 15) nkat to(9.00±0.25) nkat, then continued to rise at 72 hours while MPO activity was reduced much more in α-MSH treatment group than in ischemia group, and was(6.63 ±0.27) nkat at 24 hours. Ultrastructure observation showed that neuron was integrated in sham operated group, while nerve cellular membrane, colloid cellular membrane, and karyotheca reperfusion were damaged, mitochondrion was swollen, and ultrastructure changes reached the peak level at 12 hours in ischemia group. This was similar in the treatment group of the early stage but did not aggravated following the phase after 12 hours.CONCLUSION: α-MSH can lessen the infiltration of cerebral inflammatory cell and ICAM-1 expression following ischemia-reperfusion and has protective effects on cerebral ischemia-reperfusion damage.关键词
脑缺血/再灌注损伤/胞间粘附分子分类
医药卫生
