中国临床康复2004，Vol.8Issue(4)：786-787,2.
骨髓间充质干细胞向早期神经元样细胞诱导分化的研究
Research of the differentiation of mesenchymal stem cells into early neuron-like cells
摘要
Abstract
BACKGROUND: Mesenchymal stem cells (MSCs) can differentiate not only into interstitial cells but also into parenchyma cell( e. g. myocardial cells) .It is a hotspot of national and international researches to directionally induce MSCs to differentiate into neurocyte in vitro, which has an important theoretical significance.OBJECTIVE: To investigate the ability of MSCs to differentiate into neuron in vitro in rat.DESIGN: A randomly blank-control study.SETTING, MATERIALS AND INTERVENTIONS: Research was completed at Toxicology Department of Public Health School of Jilin University.Six-week old Wistar rats of either gender obtained from experimental animal center of Jilln University were employed. The 2nd to 4th generation of rMSCs were inoculated on 24-well plates covered by microscopic coverglass. There were 3 experiment groups and one control group. Isobutylmethylxanthine (IBMX) with a concentration of 0.25, 0.50 and 1.00 mmol/L was added respectively into 3 experiment group to induce rMSCs generation. Morphologic observation was operated after cellular differentiation. There was no inductive agent added into control group. Cellular identification was operated by immunocytochemistry.MAIN OUTCOME MEASURES: The growth of cultured MSCs; cellular morphologic changes after induction; the immunocytochemical assay of nestin (mouse antirat, monoclone), neuron-specific enolase(NSE) antibody(rabbit anti mouse, mutil-antibodies), and microtubule-associated protein-2a, b (MAP-2a, b).RESULTS: After being added with IBMX, there was little neuron-like cells differentiation in 0.25 mmol/L group; cells in 0. 1 mmol/L group gradually died since the 2nd day; neuron-like cells could be seen on the 2nd day in 0.5mmol/L group with typical morphological characteristics. 0.5 mmol/L IBMX had a best inductive effectiveness. Neuron-like cells could be seen on the 2nd day and on the 6th day, neuron-like cells were with(23.2 ± 2.3)% of the total cells, NSE dye reaction was positive. Non-differentiating cellular expression was nestin. With 3-day induction, positive cells increased, and decreased on the 6th day.CONCLUSION: rMSC can be amplified, generated in vitro and to be induced into early phase neuron-like cells.关键词
骨髓细胞/干细胞/细胞学/细胞分化分类
医药卫生引用本文复制引用
尹飞,郭丽,朱庆三,凌翎,李鹏,呼合塔娜,范洪学..骨髓间充质干细胞向早期神经元样细胞诱导分化的研究[J].中国临床康复,2004,8(4):786-787,2.基金项目
吉林省省科委资助项目(990563-1) （990563-1）
吉林省卫生厅医学科研基金项目(200131) （200131）
