首页|期刊导航|中国农业科学（英文版）|Cloning and Sequence Analysis of the gp41 Gene of Clanis bilineata Nuclear Polyhedrosis Virus
中国农业科学（英文版）2006，Vol.5Issue(10)：787-792,6.
Cloning and Sequence Analysis of the gp41 Gene of Clanis bilineata Nuclear Polyhedrosis Virus
Cloning and Sequence Analysis of the gp41 Gene of Clanis bilineata Nuclear Polyhedrosis Virus
摘要
Abstract
Clanis bilineata Nucleo Polyhedro Virus (CbNPV) was purified from Clanis bilineata larva. To obtain the molecular information of the virus, the genomic DNA of CbNPV was extracted, and a DNA fragment library of the virus was constructed using shotgun. The positive clones were then sequenced and analyzed. An open-reading frame (ORF) that has high identity with the gp41 gene of most NPVs was found in the library. The gp41 gene of CbNPV is 933 base pair long and encodes a protein of 310 amino acids. The result of the amino acid sequence analysis showed that the CbNPV gp41 has 53-61 and 56-73% identities with Group Ⅰ and Ⅱ NPVs gp41 proteins, respectively. The result indicates that the isolated CbNPV is a novel baculovirus, and the CbNPV shares a much closer relationship with Group Ⅱ NPVs.关键词
Clanis bilineata Nucleo Polyhedro Virus, gp41 gene, sequence analysisKey words
Clanis bilineata Nucleo Polyhedro Virus, gp41 gene, sequence analysis分类
农业科技引用本文复制引用
ZHU Shan-ying,WANG Wen-bing,ZHU Jiang..Cloning and Sequence Analysis of the gp41 Gene of Clanis bilineata Nuclear Polyhedrosis Virus[J].中国农业科学（英文版）,2006,5(10):787-792,6.基金项目
This work was supported by grants from the 973 National Basic Research Program of China (2005CB 121005), Agriculture Committee of Jiangsu Province (2004), and the Nature Science Foundation of Jiangsu University (04CX08). （2005CB 121005）
