首页|期刊导航|中国海洋湖沼学报（英文版）|Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD
中国海洋湖沼学报（英文版）2007，Vol.25Issue(1)：78-84,7.
Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD
Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD
摘要
Abstract
Population genetics of the left-eyed flounder, Paralichthys olivaceus, including natural and cultured stocks distributed in the coastal waters near Qingdao of eastern maritime China, was analyzed in allozyme and RAPD. The results showed that among total 29 gene loci of 15 isozymes, 9 and 7 were polymorphic in natural and cultured stocks, respectively. The status of genetic diversity in P olivaceus is low in terms of polymorphic loci in chi-square test and genetic departure index of Hardy-Weinberg equilibrium. More alleles in IDHP, CAT, GDH and Ldh-C allozymes were found in the fish, which could be used as markers in assortive breeding and distinguishing stock, population or species evolution. Total 88 and 86 RAPD bands ranging from 200 to 2 500 bp were recognized individually in average of 7.8-8.0 bands per primer. The genetic diversity in cultured stock is lower than that in natural ones showing an obviously decreasing genetic divergence. Therefore, effective countermeasures must be taken to protect genetic resources of marine cultured fishes. The 2 markers have their own pros and cons. Combining the 2 markers to investigate the genetic variation of populations is suggested. The results provide basic data of this flounder and they are useful for studying genetic improvement and genetic resources of the fish.关键词
Paralichthys olivaceus/genetic variation/natural and cultured stocks/allozyme/RAPDKey words
Paralichthys olivaceus/genetic variation/natural and cultured stocks/allozyme/RAPD分类
天文与地球科学引用本文复制引用
YOU Feng,ZHANG Peijun,WANG Keling,XIANG Jianhai..Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD[J].中国海洋湖沼学报（英文版）,2007,25(1):78-84,7.基金项目
Supported by the Natural Science Fundation of China (No. 30571445)and Natural Science Fundation of Shandong Province of China （No. 30571445）
