天然产物研究与开发2005，Vol.17Issue(6)：797-799,3.
高效液相色谱法测定辣椒干中辣椒素的含量
Determination of Capsaicin in Chilli ( Capsicum annuum L. ) by HPLC
摘要
Abstract
A simple, rapid HPLC method was adapted to determine capsaicin in chilli(Capsicum annuum L.)with Burospher-100 C18 column(I.D.250mm×4.6mm,5μm) ,unsing MeOH-H2O(70:30,V/V)as mobile phase with an eluting rate of 0.6mL/min and detected at 280mm..There is a good linear relationship in the range of 1.01～121.2mg/L for capsaicin, and the correlative coefficient is 0.9994.When the mothod was applied to analyse chilli extract, and the relative standard deviation is 1.38%(n=6)and the average recovery is 99.88%.关键词
高效液相色谱/辣椒/辣椒素/定量分析Key words
HPLC/Capsicum annuum/capsaicin/quantitative analyse分类
化学化工引用本文复制引用
高艺,钟世安,周春山,陈龙胜..高效液相色谱法测定辣椒干中辣椒素的含量[J].天然产物研究与开发,2005,17(6):797-799,3.基金项目
Project supported by the Natural Science Foundation of Hunan Province( No. 04JJ3080) （ No. 04JJ3080）
