农杆菌介导的马铃薯试管薯遗传转化体系的优化及反义class Ⅰ patatin基因的导入OA北大核心CSCDCSTPCD
An Efficient Protocol for Agrobacterium-mediated Transformation with Microtuber and the Introduction of an Antisense class Ⅰ patatin Gene into Potato
用两个马铃薯栽培品种"鄂马铃薯3号"和"甘农薯2号"的试管薯为供体材料,建立了一种农杆菌介导的简单、快速和高效的遗传转化系统.在含有75 mg/L卡那霉素的选择培养基上,2～3周可产生抗性芽,4～5周获得完整的转基因植株.筛选出了试管薯遗传转化的优化条件,特别是在再生培养基中加入2 mg/L玉米素,两个品种的转化频率分别高达45.5%和43.9%.周期短(4～5周)、一步培养和转化频率高,使该转化体系能够广泛用于马铃薯转基因的研究.用含有反义cl…查看全部>>
A simple, rapid and high efficient protocol was established for Agrobacterium-mediated transformation with microtuber of two potato cultivars, E-potato 3 and Gannongshu 2. Adventitious buds differentiation occurred after 2-3 weeks on selective medium containing 75 mg/L kanamycin, and transgenic plants could be obtained in 4-5 weeks. By combining the best treatments, especially using 2 mg/L zeatin riboside (ZR) in the shoot induction medium, this protocol yie…查看全部>>
司怀军;谢从华;柳俊
华中农业大学园艺林学学院华中农业大学生命科学技术学院,湖北武汉,430070华中农业大学园艺林学学院
农业科技
马铃薯根癌农杆菌转化试管薯反义patatin基因
PotatoAgrobacterium tumefaciensTransformationMicrotuberAntisense patatin gene
《作物学报》 2003 (6)
马铃薯试管块茎形成的分子机理及调控
801-805,5
国家自然科学基金资助项目(39970464).National Natural Science Foundation of China (39970464).
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