作物学报2001，Vol.27Issue(1)：80-84,5.
关键因子对棉花利用农杆菌介导法导入外源基因的影响
Influence of Some Factors on Transgenic Cotton by Agrobacterium-mediated Gene Transfer
摘要
Abstract
Hypocotyl segments from 5～6 day old seedlings of Grossypiumhirsutum were cocultured with Agrobacterium tumefaciens in order to introduce Bt and tfdA genes into cotton crop. GUS gene is as marker gene and NPTⅡ gene is as selecting gene. The cultures were cocultured in MS medium contained 0.1 mg/L 2,4-D and 0.1 mg/L KT for 48h, then transfered into the medium supplemented with 500 mg/L Cefotaxime and 50 mg/L Kanamycin to induce and select resistant calli. After 70～80 days, GUS gene was inspected and the positive calli were selected and cultured on embryogenesis medium until globular embryos developing and germinated plantlets forming.关键词
棉花/农杆菌/基因转化/再生植株/GUS基因分类
农业科技引用本文复制引用
李宝平,赵俊侠,石跃进,郭三堆,桑瑜,齐宏立,焦改丽,陈志贤..关键因子对棉花利用农杆菌介导法导入外源基因的影响[J].作物学报,2001,27(1):80-84,5.基金项目
本项目为国家863计划和山西省科委资助项目(DH02-01-01(Z17-01-01)) （DH02-01-01(Z17-01-01）
