中国临床康复2004，Vol.8Issue(35)：8122-8123,2.
兔牙本质磷酸蛋白稳定性及其生物学特性分析
Stability and biological characteristics of rabbit dentin phosphoprotein
摘要
Abstract
BACKGROUND: Dentin non-collagenous proteins(NCPs) participate in the mineralization of dentin matrix and the formation process of tertiary dentin. Dentin phosphoprotein(DPP), the main component of NCPs, is the key factor in stabilizing mineralized tissues. Because there are various proteolytic enzyme systems in NCPs that can potentially threaten the stability of DPP, it is presumed that there are probably some material systems in NCPs that can prevent protein degradation.OBJECTIVE: To investigate the stability and formation mechanism of DPP.DESIGN: A nonrandomized and uncontrolled study was conducted.SETTING and PARTICIPANTS: The experiment was completed in the School of Stomatology, Wuhan University. The subjects were 75 Japanese male long-eared rabbits, aged 70 - 90 days, with a body mass of 2 000 - 2 200 g (provided by Center for Experimental Animals, Chinese Academy of Sciences ).INTERVENTIONS: DPP was extracted from dentin NCPs of rabbit incisors and gel filtration and ion-exchange chromatography techniques were used to purify DPP.MAIN OUTCOME MEASURES: The inhibition of DPP degradation by various protease inhibitors.RESULTS: Calcium ion could initiate DPP degradation system, which could be inhibited by thiol protease inhibitor N-ethyl-maleimide (NEM) so that DPP degradation could be prevented.CONCLUSION: There are probably calcium ion-dependent proteolytic enzyme systems in NCPs that can promptly renovate dentin caries.关键词
兔/牙本质/蛋白质类/分析/生物降解/蛋白酶抑制剂/肽水解酶类分类
医药卫生引用本文复制引用
贺红,樊明文,边专,张旗,熊卫星..兔牙本质磷酸蛋白稳定性及其生物学特性分析[J].中国临床康复,2004,8(35):8122-8123,2.基金项目
中国卫生部基金资助(96222) （96222）
