首页|期刊导航|中国农业科学（英文版）|SNP Analysis and Haplotype Identification in Chymotrypsin Inhibitor-2 (CI-2) Gene of Barley
中国农业科学（英文版）2009，Vol.8Issue(1)：8-14,7.
SNP Analysis and Haplotype Identification in Chymotrypsin Inhibitor-2 (CI-2) Gene of Barley
SNP Analysis and Haplotype Identification in Chymotrypsin Inhibitor-2 (CI-2) Gene of Barley
摘要
Abstract
Barley chymotrypsin inhibitor-2 (CI-2) was considered to be a promising candidate for enhancing the nutritional value of other cereals by increasing its concentration as it is rich in lysine than any other storage protein. Also, it was proposed that CI-2 might play an important role in the inhibition of proteolytic enzymes from pests or pathogens as CI-2 can strongly inhibit chymotrypsin and subtilisin. In this study, a total of 93 CI-2 gene sequences were isolated from wild and cultivated barley. 48 SNPs and 4 indels were detected across the entire sequences. The frequency of SNPs in the non-coding region (1 out of 9 bases) was slightly higher than that in the coding region (1 out of 10.7 bases). In all, 33.3% of the candidate cSNPs resulted in amino acid changes. As a total, the 24 cSNPs resulted in 15 amino acid changes. Ten distinguishable haplotypes were detected, among which 3 haplotypes were shared in the most barley accessions, whereas the rest of the haplotypes appeared at a lower frequency. In addition, three haplotypes (haplotype 4, 8, and 9) were unique for single accessions. These results suggested that low diversity at the CI-2 locus was detected among the cultivated and wild barley.关键词
barley/chymotrypsin inhibitor/SNP/haplotypeKey words
barley/chymotrypsin inhibitor/SNP/haplotype分类
农业科技引用本文复制引用
ZENG Xiang-hui,WEI Yu-ming,JIANG Qian-tao,QI Peng-fei,ZHENG You-liang..SNP Analysis and Haplotype Identification in Chymotrypsin Inhibitor-2 (CI-2) Gene of Barley[J].中国农业科学（英文版）,2009,8(1):8-14,7.基金项目
This work was supported by the National Key Technology R&D Program of China (2006BAD01A02-23, 2006 BAD 13B02-06), the National High Technology Research and Development Program of China (863 Program 2006AA10Z179, 2006AA10Z1F8), and the Foundation for the Author of National Excellent Doctoral Dissertation of China (200357). （2006BAD01A02-23, 2006 BAD 13B02-06）
