国际眼科杂志2009，Vol.9Issue(5)：817-819,3.DOI:10.3969/j.issn.1672-5123.2009.05.003
胎牛血清对小鼠角膜上皮细胞生长和分化的影响
Effect of fetal bovine serum on the proliferation and differentiation of murine corneal epithelial cells in vitro
摘要
Abstract
AIM: To investigate the effect of fetal bovine serum (FBS) on the proliferation and differentiation of murine corneal epithelial cells in vitro.METHODS: Mouse corneal epithelial cells(MCEs) were cultured in serum-free low-Ca2+ medium(KSFM) and KSFM supplemented with 100mL/L FBS, respectively. Population doublings (PDs) were determined. The expressions of corneal epithelial cell markers p63, keratin 19 (K19) and involucrin were investigated by RT-PCR and Western blotting analyses. RESULTS: Cells in KSFM were stably subcultured over 25 passages; however, none of the cell lines could pass P3 in KSFM with FBS. In KSFM, the cells showed typical cobblestone appearance and expressed p63, K19 and involucrin. After medium was supplemented with FBS, cells became homogeneous, large and squamous. Furthermore, both RT-PCR and Western blotting analyses showed that the expression of involucrin was increased significantly.CONCLUSION: FBS has effects of inhibiting proliferation and triggering differentiation of MCEs.关键词
血清/角膜/上皮/细胞培养Key words
serum/cornea/epithelium/cell culture分类
医药卫生引用本文复制引用
马小力,阙艳红,孔珺,刘汉强,张劲松..胎牛血清对小鼠角膜上皮细胞生长和分化的影响[J].国际眼科杂志,2009,9(5):817-819,3.基金项目
中国辽宁省教育厅基金资助项目(No.05L564) Liaoning Provincial Department of Education, China(No.05L564) （No.05L564）
