中国现代医学杂志2006，Vol.16Issue(6)：817-821,5.
褪黑素对糖尿病大鼠肾脏病变的保护作用
Renal protective effect of melatonin in diabetic rats
摘要
Abstract
[Objectives] To investigate the mechanism of melatonin in protecting the renal lesion of diabetic rats.[Methods] Experimental diabetic rats were induced by peritoneal injection of streptozotocin. The rats were randomly divided into following groups: normal control rats (group C), streptozotocin-induced diabetic rats (group D) and diabetic rats treated with melatonin (group M). After 4 weeks, the serum creatinine, kidney hypertrophy index (kidney weight/body weight ratio), and 24 h urinary albumin excretion rate were measured, respectively. TGF-β1 mRNA expression in renal cortex of rats using newly real-time quantitative RT-PCR with SYBR Green I and the kidney samples were observed with light microscope, mean glomerular transverse sectional area (MGA) and mean glomerular volume (MGV) were also calculated by analysis system of the image. [Results] After 4 weeks, kidney hypertrophy index and 24 h urinary albumin excretion of melatonin treatment group were significantly lower than those in diabetic group D (P ＜0.01). TGF-β1 mRNA expression levels revealed a 9.2-fold increase in diabetic glomeruli after four weeks compared to normal glomeruli (P ＜0.01). While melatonin treatment decrease 3.95-fold, but still significantly higher compared to normal glomeruli (P ＜0.01). Furthermore, the MGA and MGV in group M were significantly smaller than those of group D (P ＜0.01). [Conclusion] Melatonin possesses a renal protective effect on diabetic rats,whose mechanism may be associated partly with a down-regulation of the expression of TGF-β1 mRNA.关键词
糖尿病肾病/转化生长因子-β1/实时荧光定量RT-PCRKey words
diabetic nephropathy/transforming growth factor β1/real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR)分类
医药卫生引用本文复制引用
涂晓文,陈英剑..褪黑素对糖尿病大鼠肾脏病变的保护作用[J].中国现代医学杂志,2006,16(6):817-821,5.基金项目
Youth fund of Shan Dong Province (Project Number :2001CA2EFB1) （Project Number :2001CA2EFB1）
