中国医科大学学报2001，Vol.30Issue(2)：81-82,2.
抑制C-myc基因表达对移植血管狭窄的影响
Effect of Inhibiting C-myc Gene on Graft Stenosis
张强 1王新文 1刁彦鹏 1辛世杰 1王斌 2段志泉1
作者信息
- 1. 中国医科大学第一临床学院外科,
- 2. 中国人民解放军第202医院外科
- 折叠
摘要
Abstract
Objective: To investigate the effect of inhibitng C-myc expression by actinomycin D on intimal hy-perplasia in vein graft. Methods: The vein graft model was established in rats. The different dises of actinomycin D (0.015 mg/kg; 0.15 mg/kg) were given just before and after operation. The vein grafts were harvested at 2 hrs and 1 week after grafting. C-myc mRNA was measured by in situ hybridization method. The intimal thickness was measured using a computerised image analysis system. Results: The expression of C-myc mRNA and the intimal thickness were both significantly reduced in large dose (0.15 mg/kg) group of actinomycin D, with 6.5%; 18.7 μm compared with control group in 12.5%; 28.5 μm respectively. Conclusion: Actinomycin D can inhibit expression of C-myc mRNA and intimal hyperplasia in graft. Expression of C-myc plays an important role in inducing proliferation of smooth muscle cell in vein graft.关键词
C-myc基因/放射菌素D/内膜增殖/原位杂交分类
医药卫生引用本文复制引用
张强,王新文,刁彦鹏,辛世杰,王斌,段志泉..抑制C-myc基因表达对移植血管狭窄的影响[J].中国医科大学学报,2001,30(2):81-82,2.
