中国农业科技导报2009，Vol.11Issue(3)：81-87,7.
亚硝酸还原酶高产菌株的筛选及发酵条件优化
Screening of High Nitrite Reductase Producing Strains and the Optimization of Fermentation
摘要
Abstract
The nitrite reductase production strain B.megaterium MPF-906 was treated with UV. The high nitrite reductase producing mutant Z85 was obtained and its nitrite reductase activity was 28.76 U/mL. The effects of compositions of culture medium and cultural conditions on nitrite reductase production were investigated. The optimal culture media are: glucose 1.5%, beef extract 0.2%, (NH4)2SO4 0.1%, K2HPO4 0.135%, KH2PO4 0.07%, NaCl 0.1%, MgSO4 · 7H2O 0.04%, MnSO4 · H2O 0.001%, CaCl2 · 2H2O 0.002%. The fermentation conditions are 30℃ for 20 h, 160 rpm, initial pH 6.5 and 4% of inoculation. Under this optimized conditions, the nitrite reductase activity reached 45.94 U/mL.关键词
亚硝酸还原酶/紫外诱变/发酵/条件优化Key words
nitrite reductase/ UV/ fermentation/ condition optimization分类
化学化工引用本文复制引用
郑怀忠,陈发河,李淑燕,孙君社,刘萍..亚硝酸还原酶高产菌株的筛选及发酵条件优化[J].中国农业科技导报,2009,11(3):81-87,7.基金项目
国家863计划项目(2008AA10Z306)资助. （2008AA10Z306）
