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The Special Expression and Comparison of the c-kit Protein in Spermatogenesis of Three Species of Locusts of Arcypteridae

ZHAO Zhuo XI Geng-si

中国农业科学(英文版)2007,Vol.6Issue(7):825-831,7.
中国农业科学(英文版)2007,Vol.6Issue(7):825-831,7.

The Special Expression and Comparison of the c-kit Protein in Spermatogenesis of Three Species of Locusts of Arcypteridae

The Special Expression and Comparison of the c-kit Protein in Spermatogenesis of Three Species of Locusts of Arcypteridae

ZHAO Zhuo 1XI Geng-si2

作者信息

  • 1. College of Life Sciences, Jilin Normal University, Siping 136000, P.R. China
  • 2. College of Life Sciences, Shaanxi Normal University, Xi'an 710062, P.R. China
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摘要

Abstract

The aim of this study was to elucidate the expression and regulation of the c-kit protein in spermatogenesis of locusts.Immunohistochemistry and biological statistics were used to investigate the expression of the c-kit protein in four representative phases of spermatogenesis of three dominant species of locusts of Arcypteridae (Orthoptera: Acridoidea),namely, Omocestus viridulus (Linnaeus), Euchorthippus unicolor (Ikonn.), and Euchorthippus vittatus Zheng, and so on, in Siping area of Jilin Province, China. The results revealed the following: (1) There was weak positive expression of the c-kit protein in spermatogonia and the positive granules were thinner; (2) there was a strong positive expression of the c-kit protein in primary spermatocyte and the positive granules became the largest than in all developmental stages; (3)the c-kit protein positive expression became stronger in secondary spermatocyte, while the positive granules became thinner; (4) there was strong positive expression of the c-kit protein and the positive granules were thinner in mature sperm, which were distributed on its head and tail; (5) there were strong positive protein granules massing at the end of spermary; (6) the positive intensity of the c-kit protein in spermatogenesis was significantly different among different species of locusts. The data suggested that the c-kit protein may play a crucial role in spermatogenesis as well as maintain the physiological action of sperms and fertilization, regulate the developmental speed of spermatogenesis, and/or maintain species isolation, etc.

关键词

locust/spermatogenesis/c-kit protein/special expression/significant difference

Key words

locust/spermatogenesis/c-kit protein/special expression/significant difference

分类

农业科技

引用本文复制引用

ZHAO Zhuo ,XI Geng-si..The Special Expression and Comparison of the c-kit Protein in Spermatogenesis of Three Species of Locusts of Arcypteridae[J].中国农业科学(英文版),2007,6(7):825-831,7.

基金项目

This research was supported by the Natural Science Foundation of Shaanxi Province, China (2004C115). (2004C115)

中国农业科学(英文版)

2095-3119

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